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glycolytic fiber  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank glycolytic fiber
    Glycolytic Fiber, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 98/100, based on 845 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glycolytic+fiber/anti-Myosin+heavy+chain+Type+IIB/pm40419762-381-53-64
    Average 98 stars, based on 845 article reviews
    glycolytic fiber - by Bioz Stars, 2026-10
    98/100 stars

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    Staining:

    Article Title: Skeletal muscle endothelial dysfunction through the activin A–PGC1α axis drives progression of cancer cachexia
    Article Snippet: .. Fiber type staining was performed as described previously , using the 5-μm cross-cryosections of GC or TA muscles with myofiber type-specific primary antibodies: type 1 for slow oxidative fiber with BA-D5 (antibody isotype MIgG2b, 1:50 dilution), type 2a for fast oxidative fiber with sc-71 (antibody isotype MIgG1, 1:100 dilution) and type 2b for fast glycolytic fiber with BF-F3 (antibody isotype MIgM, 1:50 dilution) antibody from DSHB for overnight at 4 °C, followed by staining with goat anti-mouse IgG2b Alexa Fluor546 secondary antibody (A-21143, 1:400 dilution), rat anti-mouse IgG1–FITC secondary antibody (11–4015-82, 1:400 dilution), goat anti-mouse IgM (heavy chain) Alexa Fluor 633 secondary antibody (A21046, 1:400 dilution) or goat anti-mouse IgM DyLight650 secondary antibody (SA5–10153, 1:400 dilution) for 1 h at room temperature. ..

    Article Title: Skeletal muscle endothelial dysfunction through the activin A-PGC1α axis drives progression of cancer cachexia.
    Article Snippet: .. Fiber type staining was performed as described previously34,79 using the 5-μm cross-cryosections of GC or TA muscles with myofiber type-specific primary antibodies: type 1 for slow oxidative fiber with BA-D5 (antibody isotype MIgG2b, 1:50 dilution), type 2a for fast oxidative fiber with sc-71 (antibody isotype MIgG1, 1:100 dilution) and type 2b for fast glycolytic fiber with BF-F3 (antibody isotype MIgM, 1:50 dilution) antibody from DSHB for overnight at 4 °C, followed by staining with goat anti-mouse IgG2b Alexa Fluor546 secondary antibody (A-21143, 1:400 dilution), rat anti-mouse IgG1–FITC secondary antibody (11-4015-82, 1:400 dilution), goat anti-mouse IgM (heavy chain) Alexa Fluor 633 secondary antibody (A21046, 1:400 dilution) or goat anti-mouse IgM DyLight650 secondary antibody (SA5-10153, 1:400 dilution) for 1 h at room temperature. .. We purchased C57BL/6J (000664) or BALB/cJ (000651) mice from Jackson Laboratory as control mice.

    Muscles:

    Article Title: Skeletal muscle endothelial dysfunction through the activin A–PGC1α axis drives progression of cancer cachexia
    Article Snippet: .. Fiber type staining was performed as described previously , using the 5-μm cross-cryosections of GC or TA muscles with myofiber type-specific primary antibodies: type 1 for slow oxidative fiber with BA-D5 (antibody isotype MIgG2b, 1:50 dilution), type 2a for fast oxidative fiber with sc-71 (antibody isotype MIgG1, 1:100 dilution) and type 2b for fast glycolytic fiber with BF-F3 (antibody isotype MIgM, 1:50 dilution) antibody from DSHB for overnight at 4 °C, followed by staining with goat anti-mouse IgG2b Alexa Fluor546 secondary antibody (A-21143, 1:400 dilution), rat anti-mouse IgG1–FITC secondary antibody (11–4015-82, 1:400 dilution), goat anti-mouse IgM (heavy chain) Alexa Fluor 633 secondary antibody (A21046, 1:400 dilution) or goat anti-mouse IgM DyLight650 secondary antibody (SA5–10153, 1:400 dilution) for 1 h at room temperature. ..

    Article Title: Skeletal muscle endothelial dysfunction through the activin A-PGC1α axis drives progression of cancer cachexia.
    Article Snippet: .. Fiber type staining was performed as described previously34,79 using the 5-μm cross-cryosections of GC or TA muscles with myofiber type-specific primary antibodies: type 1 for slow oxidative fiber with BA-D5 (antibody isotype MIgG2b, 1:50 dilution), type 2a for fast oxidative fiber with sc-71 (antibody isotype MIgG1, 1:100 dilution) and type 2b for fast glycolytic fiber with BF-F3 (antibody isotype MIgM, 1:50 dilution) antibody from DSHB for overnight at 4 °C, followed by staining with goat anti-mouse IgG2b Alexa Fluor546 secondary antibody (A-21143, 1:400 dilution), rat anti-mouse IgG1–FITC secondary antibody (11-4015-82, 1:400 dilution), goat anti-mouse IgM (heavy chain) Alexa Fluor 633 secondary antibody (A21046, 1:400 dilution) or goat anti-mouse IgM DyLight650 secondary antibody (SA5-10153, 1:400 dilution) for 1 h at room temperature. .. We purchased C57BL/6J (000664) or BALB/cJ (000651) mice from Jackson Laboratory as control mice.



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